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KMID : 1094720170220010089
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Biotechnology and Bioprocess Engineering
2017 Volume.22 No. 1 p.89 ~ p.94
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Bacterial cellulose-chitosan composite hydrogel beads for enzyme immobilization
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Kim Hyun-Jung
Jin Ju-Nam
Kan Eun-Sung
Kim Kwang-Jin
Lee Sang-Hyun
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Abstract
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In this work, we report the preparation of bacterial cellulose (BC)-chitosan composite hydrogel beads by co-dissolution of BC and chitosan in 1-ethyl-3-methylimidazolium acetate and subsequent reconstitution with distilled water. The BC-chitosan hydrogel beads were used as enzyme supports for immobilizing Candida rugosa lipase by physical adsorption and covalent cross-linking. BC-chitosan hydrogel beads immobilized lipase more efficiently than microcrystalline cellulose (MCC)-chitosan hydrogel beads. The amount of protein adsorbed onto BCchitosan beads was 3.9 times higher than that adsorbed onto MCC-chitosan beads, and the catalytic activity of lipase was 1.9 times higher on the BC-chitosan beads. The lipase showed the highest thermal and operational stability when covalently cross-linked on BC-chitosan hydrogel beads. The half-life time of the lipase cross-linked on BC-chitosan bead at 60°C was 22.7 times higher than that of free lipase. Owing to their inherent biocompatibility and biodegradability, the BC-chitosan composite hydrogel beads described here could be used to immobilize proteins for various biomedical, environmental, and biocatalytic applications.
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KEYWORD
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bacterial cellulose, chitosan, hydrogel bead, lipase, immobilization
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